Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies

• Xuefeng Wang,
• Zhong Mei,
• Yanyan Wang and
• Liang Tang

Beilstein J. Nanotechnol. 2017, 8, 372–380, doi:10.3762/bjnano.8.39

• GNR biofunctionalization and can be easily extended to other sensing applications based on other gold nanostructures or new biomolecules. Keywords: biofunctionalization; biosensing; four methods; gold nanorod; introduction of sulfhydryl groups; Introduction Gold nanorods (GNRs) are widely used in

• , the antibody activity would not be affected because the antigen-binding site is not modified by DTT reduction [19][20]. The introduction of sulfhydryl groups through easy surface modifications by Traut’s reagent and DTT can be employed for the covalent conjugation of GNRs through the formation of a

• methods for the biofunctionalization of GNRs through the introduction of sulfhydryl groups to antibodies by using Traut’s reagent, DTT, PEG6-CONHNH2, and SH-PEG-NH2. We compared the sensitivity and specificity to detection of human IgG targets of four thiolated antibodies by using a functionalized-GNR